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gene chip hybridization oven 320  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene chip hybridization oven 320
    Gene Chip Hybridization Oven 320, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+chip+hybridization+oven+320/pmc04900233-75-0-5
    Average 90 stars, based on 1 article reviews
    gene chip hybridization oven 320 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells.
    Article Snippet: Lung Cancer (2004) 43, 17—28 Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells John Novak, Shea Schleman, Jennifer Scott, Valerie L. Balderman, Laura Krech, Madeleine A. Kane* Section of Medical Oncology, Denver Veterans Affairs Medical Center, University of Colorado Health Sciences Center and University of Colorado Cancer Center, 1055 Clermont Street, Denver, CO 80220, USA Received 3 April 2003 ; received in revised form 7 July 2003; accepted 17 July 2003 KEYWORDS Bombesin; Gastrin-releasing peptide; Receptors; Glucocorticoid; Lung cancer; Human; Regulation Summary We investigated the effects of the glucocorticoid, dexamethasone (Dex), on expression of the gastrin-releasing peptide (GRP) receptor by human small cell lung carcinoma (SCLC) SHP77 cells.. After 12 h of 10 nM Dex exposure, a six-fold increase in the peak of GRP receptor mRNA compared with untreated controls (10.5± 4 versus 1.65± 0.15 attomols/ g total RNA, respectively, P < 0.05) occurred.. GRP receptor mRNA levels fell to less than 0.5 attomols/ g total RNA after 24 h; in Dex-treated cells, these levels rose to 1.2 compared with 0.12 attomols/ g total RNA in the absence of Dex after 7 days.

    Hybridization:

    Article Title: Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells.
    Article Snippet: Lung Cancer (2004) 43, 17—28 Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells John Novak, Shea Schleman, Jennifer Scott, Valerie L. Balderman, Laura Krech, Madeleine A. Kane* Section of Medical Oncology, Denver Veterans Affairs Medical Center, University of Colorado Health Sciences Center and University of Colorado Cancer Center, 1055 Clermont Street, Denver, CO 80220, USA Received 3 April 2003 ; received in revised form 7 July 2003; accepted 17 July 2003 KEYWORDS Bombesin; Gastrin-releasing peptide; Receptors; Glucocorticoid; Lung cancer; Human; Regulation Summary We investigated the effects of the glucocorticoid, dexamethasone (Dex), on expression of the gastrin-releasing peptide (GRP) receptor by human small cell lung carcinoma (SCLC) SHP77 cells.. After 12 h of 10 nM Dex exposure, a six-fold increase in the peak of GRP receptor mRNA compared with untreated controls (10.5± 4 versus 1.65± 0.15 attomols/ g total RNA, respectively, P < 0.05) occurred.. GRP receptor mRNA levels fell to less than 0.5 attomols/ g total RNA after 24 h; in Dex-treated cells, these levels rose to 1.2 compared with 0.12 attomols/ g total RNA in the absence of Dex after 7 days.

    Article Title: The Cxcl12, Periostin, and Ccl9 Genes Are Direct Targets for Early B-cell Factor in OP-9 Stroma Cells
    Article Snippet: .. 20 g of the final fragmented cRNAwas then hybridized to Affymetrix chips U74Av2 or MOE4302 (Affymetrix) in 200 l of hybridization buffer (100 mMMES buffer, pH 6.6, 1 M NaCl, 20 mM EDTA, 0.01% Tween 20) supplemented with herring sperm DNA (100 g/ml) and acetylated bovine serum albumin (500 g/ml) in an Affymetrix Gene ChIP Hybridization oven 320. .. The chip was then developed by the addition of fluorescein isothiocyanate-streptavidin followed by washing using an Affymetrix Gene Chip Fluidics Station 400.

    Article Title: Nicotine Exposure During Pregnancy Results in Persistent Midline Epithelial Seam With Improper Palatal Fusion
    Article Snippet: RNA samples were profiled using Affymetrix GeneChip Mouse Genome (MG) 430 2.0 arrays (Platform: {"type":"entrez-geo","attrs":{"text":"GPL11180","term_id":"11180"}} GPL11180 , Affymetrix, Inc, Santa Clara, CA) according to the standard Affymetrix gene chip analysis protocol. .. Gene Chip Hybridization Oven 320 (Affymetrix) and Gene Chip Fluidics Station 400 (Affymetrix) at UNMC Microarray Core were used for hybridization and scanning. ..

    Article Title: RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development.
    Article Snippet: The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns.. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell lines representing defined stages of B cell development using Affymetrix microarrays.. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell lines was established.

    Article Title: Microarray analysis of gene expression during epithelial-mesenchymal transformation.
    Article Snippet: Each 40- l fragmented sample, along with the corresponding murine U74A gene chips (Affymetrix), was then submitted to an Affymetrix core facility, where hybridization of the RNA to the chip probes and fluidics (washing and staining) were completed using the standard Affymetrix gene chip analysis protocol. .. Sample processing was completed using the Gene Chip Hybridization Oven 320 (Affymetrix) and Gene Chip Fluidics Station 400 (Affymetrix). .. Completed chips were analyzed for expression intensity using an Affymetrix Gene Array Scanner (Hewlett-Packard).

    Gene Expression:

    Article Title: Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells.
    Article Snippet: Lung Cancer (2004) 43, 17—28 Dexamethasone regulation of gastrin-releasing peptide receptor in human lung cells John Novak, Shea Schleman, Jennifer Scott, Valerie L. Balderman, Laura Krech, Madeleine A. Kane* Section of Medical Oncology, Denver Veterans Affairs Medical Center, University of Colorado Health Sciences Center and University of Colorado Cancer Center, 1055 Clermont Street, Denver, CO 80220, USA Received 3 April 2003 ; received in revised form 7 July 2003; accepted 17 July 2003 KEYWORDS Bombesin; Gastrin-releasing peptide; Receptors; Glucocorticoid; Lung cancer; Human; Regulation Summary We investigated the effects of the glucocorticoid, dexamethasone (Dex), on expression of the gastrin-releasing peptide (GRP) receptor by human small cell lung carcinoma (SCLC) SHP77 cells.. After 12 h of 10 nM Dex exposure, a six-fold increase in the peak of GRP receptor mRNA compared with untreated controls (10.5± 4 versus 1.65± 0.15 attomols/ g total RNA, respectively, P < 0.05) occurred.. GRP receptor mRNA levels fell to less than 0.5 attomols/ g total RNA after 24 h; in Dex-treated cells, these levels rose to 1.2 compared with 0.12 attomols/ g total RNA in the absence of Dex after 7 days.

    Chromatin Immunoprecipitation:

    Article Title: The Cxcl12, Periostin, and Ccl9 Genes Are Direct Targets for Early B-cell Factor in OP-9 Stroma Cells
    Article Snippet: .. 20 g of the final fragmented cRNAwas then hybridized to Affymetrix chips U74Av2 or MOE4302 (Affymetrix) in 200 l of hybridization buffer (100 mMMES buffer, pH 6.6, 1 M NaCl, 20 mM EDTA, 0.01% Tween 20) supplemented with herring sperm DNA (100 g/ml) and acetylated bovine serum albumin (500 g/ml) in an Affymetrix Gene ChIP Hybridization oven 320. .. The chip was then developed by the addition of fluorescein isothiocyanate-streptavidin followed by washing using an Affymetrix Gene Chip Fluidics Station 400.

    other:

    Article Title: The CD53 and CEACAM-1 genes are genetic targets for early B cell factor.
    Article Snippet: The final fragmented cRNA (20 lg) was then hybridized to Affymetrix chip U74Av2 (Affymetrix) in 200 lL hybridization buffer (100 mMMES buffer pH 6.6, 1 M NaCl, 20 mM EDTA, 0.01% Tween-20) supplemented with Herring sperm DNA (100 lg/mL) and acetylated BSA (500 lg/mL) in an Affymetrix Gene Chip Hybridization oven 320.

    Article Title: Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics.
    Article Snippet: Åkerblad, Peter, Robert Månsson, Anna Lagergren, Simonetta Westerlund, Barbro Basta, Ulrika Lind, Anders Thelin, Ramiro Gisler, David Liberg, Sven Nelander, Krister Bamberg, and Mikael Sigvardsson.. Gene expression analysis suggests that EBF-1 and PPAR 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics.. Physiol Genomics 23: 206–216, 2005.

    Microarray:

    Article Title: Nicotine Exposure During Pregnancy Results in Persistent Midline Epithelial Seam With Improper Palatal Fusion
    Article Snippet: RNA samples were profiled using Affymetrix GeneChip Mouse Genome (MG) 430 2.0 arrays (Platform: {"type":"entrez-geo","attrs":{"text":"GPL11180","term_id":"11180"}} GPL11180 , Affymetrix, Inc, Santa Clara, CA) according to the standard Affymetrix gene chip analysis protocol. .. Gene Chip Hybridization Oven 320 (Affymetrix) and Gene Chip Fluidics Station 400 (Affymetrix) at UNMC Microarray Core were used for hybridization and scanning. ..



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    Thermo Fisher gene chip hybridization oven 320
    Gene Chip Hybridization Oven 320, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+chip+hybridization+oven+320/pmc04900233-75-0-5
    Average 90 stars, based on 1 article reviews
    gene chip hybridization oven 320 - by Bioz Stars, 2026-09
    90/100 stars
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